Immunohistochemical expression of CD44
in thyroid gland lesions
Kiziridou A.1, Pantidou A.2,
Papayianni M.1, Destouni Ch.2,
Technician: Velkopoulou E.
1Department of Pathology
2Department of Cytopathology,
“Theagenio” Anticancer Hospital of Thessaloniki
Aim: CD44 is a polymorphic family of cell surface proteoglycans
and glycoproteins implicated in cell-to-cell and cell-to-matrix
adhesion interactions and tumor metastasis. Its expression appears
to be an indicator of invasive and metastatic behaviour in carcinomas.
The purpose of our study is to investigate the immunohistochemical
expression of CD44 protein in thyroid lesions and its association
to other histopathological parameters.
Material and Methods: Samples from thyroid lesions were
obtained from 40 patients treated in our Hospital. The material
consisted of 5 cases of multinodular goiter, 7 cases of thyroiditis
Hashimoto, 5 cases of follicular adenoma, 4 cases of Hurthle cell
tumor, 15 cases of thyroid carcinoma (11 papillary carcinomas,
and 4 myeloid tumors), and 4 cases of normal thyroid tissue. Immunostaining
was performed using the Ventana ES automated immunostainer. A
monoclonal antibody was used and avidin-biotin method was applied
to paraffin-embedded samples. A membranous immunostaining pattern
was considered positive.
Results: CD44 expression was detected in three adenomas
(60%), mostly of follicular type, and in 8 carcinomas (72%). The
CD44 immunostaining was especially apparent in papillary type
of carcinomas, which showed high expression. In normal thyroid
tissue, a reduced CD44 expression was observed.
Conclusions: The results of our study indicate that deregulated
expression of CD44 contributes to the ability of thyroid carcinomas
for invasion and metastasis and may constitute a prognostic factor
for malignant biological behaviour.
Key words: CD44, immunohistochemistry, thyroid gland.